Evaluation of Salmonella abortusovis detection by primers related to serovar specific IS200

ABSTRACT

Evaluation of primers designed due to theserovar specific IS200 copy for detecting Salmonellaabortusovis strains isolated in Iran. Observational study. Ninety seven Salmonella abortusovis strains. PCR amplification was carried out by serovarspecific primers and different strains according to PCRresults were studied by IS200 fingerprinting analysis. All strains could be classified in 2 distinctgenotypes by 2 kb and 900 bp amplicons in PCRamplification. These two genotypes were related to twodifferent profiles with 11 and 9 kb band respectively in IS200 fingerprinting. PCR amplification by serovar specificprimers was capable of grouping the strains in 2 majorgenotypic patterns