Effect of various levels of probiotic on morphology of intestinal mucosa in brioler chicks

ABSTRACT

To Investigate the effect of probiotic on morphology of small intestine in roosters. Factoriel 4 x 6 in completely randomized design. 240 male broilers of Ross commecial hybrid designated into 4 groups [N= 60 chicks]. Experimental groups were fed diet with different levels of probiotic [Bioplus 2B] from day 1-42.Treatment were done with just diet [basal or 0%] and with increasing percentages of probiotic [0.05, 0.01 and 0.15%].Different segments of small intestine [1,10, 30,50, 70 and 90% of total length] were extracted from each group [n=5] and analyzed for villus indices [hight [H], width [W], H/W ratio], depth of cripts and villus types [tongue shape, leaf shape, finger like, bridge from, ridged and convoluted] were recorded. Data were analyzed through ANOVA by MSTATC and means of different villus parameters were compared by Duncans Multiple test. While intake of varius levels of probiotic significantly increased villus height in the first part of small intestine above basal levels [P<0.05], its width had any change. H/W ratio was not affected by the probiotic intake. Despite an apparent increase of crypt depth in the first and last segments of small intestine [P<0.05] probiotic intake did not change it in the mid portion of the intestine. Percentage of different types of villi were not affected by consumption of probiotic. Administration of probiotic in poultry diets increases villus height of the first segment and crypt depth in the first and last parts of small intestine. These data suggest that probiotic administration providing increased food digestion and absorption in chickens