association between single nucleotide polymorphism in interleukin-27 gene and recurrent pregnancy loss in Iranian women

ABSTRACT

Recurrent pregnancy loss [RPL] has been defined as two or more miscarriages before 20[th] week of gestation. It seems that IL-27 may reduce inflammatory responses and affect the survival of the embryo during human pregnancy. IL-27 polymorphisms may influence RPL by altering the levels or the activity of gene product. We studied for the first time the association of IL-27 -964 A>G single nucleotide polymorphism [SNP] with RPL in Iranian women. A case-controlled study was performed on two groups consisting of 150 healthy women with at least one delivery [control group] and 150 women with two or more primary RPLs history [RPL group]. The -964 A>G SNP in IL-27 gene was determined by PCR-RFLP technique. Genotype and allele frequencies were compared using Chi[2] tests between two groups. There was no difference between the two groups regarding age of women [29 +/- 4.4 [control] vs. 30.84 +/- 5.2 years [case]]. In the RPL group, the genotype frequencies of -964 A>G polymorphism were AG [49.3%], AA [40%], and GG [10.7%], and in the control group, they were AG [43.3%], AA [48.7%], and GG [8%]. There was no significant difference between the genotypes of AA, AG, and GG in two groups [p=0.23]. As the frequency of allele A was 64.7% in the RPL group and 70.3% in the control group, the difference in frequency of allele A in -964 A>G between two groups was not significant [p=0.19]. Our findings indicate that SNP of -964 A>G in IL-27 gene is not a risk factor for RPL in Iranian women