[Evaluation of the effect of clomiphen citrate on osteoporosis in the dog model: biomechanical and histopathological studies]

ABSTRACT

To evaluate the short term effect of clomiphen citrate on osteoporosis in dog. Experimental study. Ten dogs of both sexes with average weight of 23 +/- 5.6 Kg, were randomly assigned to either control [n=5] or experimental groups [n=5]. Osteoporosis was induced by immobilizing the right hindlimb by means of fiberglass cast. Five dogs received clomiphen citrate [5 mg/kg per os] once a day for 42 days. All dogs were euthanized on day 42 and tibias were harvested. Bone biomechanical properties and microscopic structure of casted and uncasted tibias were studied. Independent t-test was done for biomechemical values. Significant differences in the values of examined mechanical properties were found between controt and clomiphen - treated dogs. [p<0.05] No remarkable histopathological changes were detected in the tibias of casted limb in Clomiphen-treated dogs. These findings indicate that short term clomiphen therapy may have promising effects on prevention of osteoporosis in dog