Association between gene polymorphisms of seven newly identified loci and type 2 diabetes and the correlate quantitative traits in Chinese dong populations

ABSTRACT

There are much heterogeneity in the genetic variation of type 2 diabetes [T2D].The purpose of this study was to investigate the association of seven novel genetic loci identified in a recent genome-wide association studies [GWAS] with T2D in Chinese Dong populations. A case-controlled study was performed in individuals of Chinese Dong nationality. The genotypes of PARD3B [rs849230], LOC729993 [rs149228], EPHA4 [rs16862811], HNT [rs3099797], PTPRD [rs17584499 and rs649891], TOMM7 [rs2240727] genes were determined using Multiplex PCR-SNaPshot. The independent association between each polymorphism and T2D was assessed using unconditional binary logistic regression analysis [BLR]. A total of 136 cases of T2D and 136 control subjects were enrolled in the study. The polymorphism of rs2240727 in TOMM7 gene was associated with T2D [odds ratio [OR] = 1.65, per copy of the risk T allele, P = 0.004]. In addition, CT and TT were risk genotypes for T2D [OR [95% CIs]2.64 [1.28- 5.45] and 3.42 [1.58- 7.41] respectively]. After correcting for multiple testing, the above results remained significant [all P < 0.05]. After adjusting for the confounders of age, gender, and BMI, the association between T2D and rs2240727 remained significant [P < 0.01]. There were significantly statistical difference in levels of fasting plasm glucose[FPG] among genotypes of rs2240727 in controls and patients, the levels of FPG were significantly higher in CT and TT genotypes than in CC genotype in both groups[all P < 0.05]. The rs2240727 genetic variant in TOMM7 was associated with T2D of Chinese Dong individuals, and might enhance the risk of T2D by affecting the level of FPG