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UV spectrophotometric determination and validation of hydroquinone in liposome
IJPR-Iranian Journal of Pharmaceutical Research. 2015; 14 (2): 473-478
in English | IMEMR | ID: emr-167952
ABSTRACT
The method has been developed and validated for the determination of hydroquinone in liposomal formulation. The samples were dissolved in methanol and evaluated in 293 nm. The validation parameters such as linearity, accuracy, precision, specificity, limit of detection [LOD] and limit of quantitation [LOQ] were determined. The calibration curve was linear in 1-50 microg/mL range of hydroquinone analyte with a regression coefficient of 0.9998. This study showed that the liposomal hydroquinone composed of phospholipid [7.8%], cholesterol [1.5%], alpha ketopherol [0.17%] and hydroquinone [0.5%] did not absorb wavelength of 293 nm if it diluted 500 times by methanol. The concentration of hydroquinone reached 10 microg/mL after 500 times of dilution. Furthermore, various validation parameters as per ICH Q2B guideline were tested and found accordingly. The recovery percentages of liposomal hydroquinone were found 102 +/- 0.8, 99 +/- 0.2 and 98 +/- 0.4 for 80%, 100% and 120% respectively. The relative standard deviation values of inter and intra-day precisions were <%2. LOD and LOQ were 0.24 and 0.72 microg/mL respectively
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Index: IMEMR (Eastern Mediterranean) Main subject: Spectrophotometry, Ultraviolet / Validation Studies as Topic / Liposomes Language: English Journal: Iran. J. Pharm. Res. Year: 2015

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Index: IMEMR (Eastern Mediterranean) Main subject: Spectrophotometry, Ultraviolet / Validation Studies as Topic / Liposomes Language: English Journal: Iran. J. Pharm. Res. Year: 2015