IJPR-Iranian Journal of Pharmaceutical Research. 2015; 14 (Supp.): 43-49
in English
| IMEMR
| ID: emr-167977
ABSTRACT
In this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box-Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column [30 [degree]C] using a mobile phase consisting of ammonium acetate 0.05 M buffer/ Methanol mixture [3070] and a flow-rate of 1.0 mL/min. The specificity, linearity, precision, accuracy, LOD and LOQ of the method were validated. The method was linear over the range of 5-500 microg/mL with an acceptable correlation coefficient [r[2] = 0.9996]. The method's limit of detection [LOD] and quantification [LOQ] were 0.01 and 0.025 microg/mL respectively. The results indicate that this validated method can be used as an alternative method for assay of nystatin
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Pharmaceutical Preparations
/
Chromatography, High Pressure Liquid
Language:
English
Journal:
Iran. J. Pharm. Res.
Year:
2015
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