Frequency and sensitivity of micro-organism in post-operative wound infections: a quest for microbes

ABSTRACT

To determine the frequency of common organisms involved in post- operative wound infection. To determine the sensitivity of micro-organism for different antibiotics. Prospective cross-sectional descriptive study. This study was carried out at Surgical Units of Civil Hospital Karachi from April to September, 2010. 72 swab samples were collected from patients who had undergone operations. Samples were obtained from the hospital and processed in Civil Hospital Karachi lab [Microbiology Department]. Out of 72 bacterial isolates found in post-operative wound infection, 30 [41.66%] were E.Coli, followed by Klebsiella species 14 [19.44%], pseudomonas aeruginosa 13 [1.38%], staphylococcus aureus 6 [9.72%], providential species 1 [1.38%], proteus mirabilis 1 [1.38%], Actinobacter 1 [1.38%] and no growth 6 [9.72%]. The result showed that the occurrence of infection was higher in the age group between 20 -40 than any other group. There is no significant difference between male and female sexes in the occurrence of infection. Infection was more in the operation done under emergency circumstance than the elective ones.There was no significant association between infection, and co-morbidities and past history of wound infection. The sensitivity pattern of 4 main bacteria, Frequently found in the study i.e. E.coli, klebsiella, pseudomonas aeruginosa and s.aureus isolates, suggested that the organisms were more sensitive to imipenem, Amikacin sulphate, Ceftazidime, Cefperazone/Sulbactum and Pipercillin/Tazobactum than other groups of drugs. The most common causative organism was E.coli, infection rate was higher among the patients operated in emergency and imipenem, Amikacin sulphate were found to be more sensitive