Erythropoietin prevention effect on induced apoptosis by ischemia- reperfusion in myocytes of rat

ABSTRACT

Ischemia- reperfusion is the common cause of apoptosis in most of cells specially myocytes. Prevention and reduction of apoptosis in myocardium can be one of the main medical goal before surgical operation, angioplasty and after infarction. Erythropoietin receiving effect 24 hours before hypoxia beginning on myocytes apoptosis rate and inflammatory process following half an hour hypoxia and 1.5 hour reperfusion are aim of this study. 40 Rats were divided randomly into two groups. 24 hours before surgical operation, 5000 Iu/Kg erythropoietin was injected to experimental group. During operation 12 rats from experimental group and 11 rats from control group were lost. After anesthesia, using ligation in left coronary artery for 30 minutes hypoxia and 1.5 hours reperfusion were applied. Then Thorax was opened and after bleeding, the animal's heart was isolated and two tissue samples of infarct and non-infarct area were separated and fixed. Then blood serum samples separated and incubated in -76[degree] C. Apoptosis intensity in heart tissue was measured by tunel method CK-MB level by method and DGKC, hsCRP by Elisa using Immunodiagnostic kit. The results were calculated Mean +/- SD. Then using paired student's t- test their deference were shown. Level of statistical significant was considered P< 0.05. Activity level of CK-MB [1550U/L to 340] in experimental group was less than control group [Pserum rate was 450 and 225 ng/ml in control and experimental group respectively [PApoptosis index in infarct area was 11.5 and 4.8% in control and experimental group respectively [P<0.000]. This deference was seen in non-infarct area. 5000 IU/Kg injection of erythropoietin before ischemia reperfusion, reduce cell injury and control inflammation process in hypoxia and control myocytes apoptosis in infarct and non-infarct area