Simultaneous determination of olanzapine and fluoxetine hydrochloride in capsules by spectrophotometry, TLC-spectrodensitometry and HPLC
Journal of Advanced Research. 2013; 4 (2): 173-180
in English
| IMEMR
| ID: emr-168519
ABSTRACT
This paper describes sensitive, accurate and precise spectrophotometric, TLC-spectrodensitometric and high performance liquid chromatographic [HPLC] methods for simultaneous determination of olanzapine and fluoxetine HCl. Two spectrophotometric methods were developed, namely; first derivative [D[1]] and derivative ratio [DD[1]] methods. The TLC method employed aluminum TLC plates precoated with silica gel GF[254] as the stationary phase and methanol tolueneammonia [730.1, by volume] as the mobile phase, where the chromatogram was scanned at 235 nm. The developed HPLC method used a reversed phase C18 column with isocratic elution. The mobile phase composed of phosphate buffer pH 4.0acetonitriletriethylamine [53470.03, by volume] at flow rate of 1.0 mL min[-1]. Quantitation was achieved with UV detection at 235 nm. The methods were validated according to the International Conference on Harmonization [ICH] guidelines. The selectivity of the proposed methods was tested using laboratory-prepared mixtures. The developed methods were successfully applied for the determination of olanzapine and fluoxetine HCl in bulk powder and combined capsule dosage form
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Spectrophotometry
/
Fluoxetine
/
Chromatography, High Pressure Liquid
/
Chromatography, Thin Layer
Language:
English
Journal:
J. Adv. Res.
Year:
2013
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