Prevalence of campylobacter jejuni and coli in sheep carcasses by using cultural and PCR methods

ABSTRACT

Campylobacter species are common bacterial pathogens causing gastroenteritis in humans worldwide. A total of 148 randomly sheep carcasses were sampled by surface section of neck meat taken immediately after slaughter analyzed using microbiological examinations. Campylobacter spp. was isolated from 10.13% meat cultures samples examined. Among these 80% sample were C. jejuni and 20% sample were C. coli. Using PCR assays, the number of positive campylobacters increased to 11.48%. Of these positive samples, 82.35% were C. jejuni and 17.65% were C. coli. Significantly higher prevalence rates of Campylobacter spp. [p<0.05] were found in the meat samples taken in summer [47.05%]. The PCR is a reliable and sensitive method which can be used as a diagnostic technique for the detection of campylobacter in lamb samples