Evaluation of antibacterial activity of satureja khuzestanica J. essential oil against standard and isolated strains of listeria monocytogenes

ABSTRACT

The purpose of this study was to determine antibacterial activity of essential oil of Satureja khuzestanica against Listeria monocytogenes [PTCC1295] and strains isolated from breast milk show that. In this descriptive-analytic study, Essence of leave's plant was extracted and identified its compounds and then carvacrol was isolated. Antibacterial activities were examined by agar dilution method against L. monocytogenes. Minimum inhibitory concentration [MIC] and minimum bacterial concentration [MBC] were carried out by micro dilution method. Then bacterial suspension injected the BALB/c mice. Forty-eight h after seeing the listeriosis disease signs were started the treatment. Ampicillin [10 micro g/disc] and trimethoprim [5 g] were used as controls. The results showed that the inhibitory zone diameter standard and essential oils for strains isolated species were respectively 59 and 50 mm. This amount was determined by carvacrol, respectively, 60 and 48 mm. Inhibition zone diameter measurements for standard strains of ampicillin and trimethoprim tedious strains, respectively, 21, 40, 18 and 33 mm, respectively. The minimum inhibitory concentration of essential oils, carvacrol and ampicillin than standard strains, respectively 1.56, 1.56 and 155x10[-8] micro g/mL and MBC 3.125, 3.125 and 125x10[-7] micro g/mL was determined by the ratio of the strain 3.125, 3.125 and 0.0062 micro g/mL and MBC was 6.25, 6.25 and 0.025 micro g/mL. This study showed that bacterial cleansing properties of essential oil of this plant have a strong and effective combination that is carvacrol