Administration of three natural products as protective agents against the genotoxic and cytotoxic effect of methotrexate in mice [mus musculus]

ABSTRACT

Methotrexate is an antimetabolite and antifolate drug. It is used in treatment of cancer, autoimmune diseases. 210 healthy adult male Swiss albino mice [Mus musculus], were allotted among three groups. The animal were given daily [0.065mg/ml] of methotrexate intraperitonealy followed by injection of Omega-3 plus at dose [8.58 mg/20g mice], Apple at dose [1.43 mg/20 g mice] and Psyllium at dose [18.2mg /20 g mice] for five days. Various Structural and numerical chromosomal aberrations in bone marrow cells; mitotic activity and sperm head abnormality were recorded, quantitated, and statistically analyzed. Also DNA extraction and apoptosis detection in liver was done. The intensity of apoptotic bands located at 200 b p; 400b p ; 600 b p;800 b p and intact DNA measured by software Gel Pro program as maximum optical density values. Methotrexate had adverse effect on chromosomal and sperm head structure, also it induce apoptosis, necrosis and decrease total DNA in mice liver.Omega-3 plus, Apple, and Psyllium attenuate the methotrexate-related toxic effects