Cytotoxicity and oxidative stress of arsenic and the possible ameliorative effect of Coriandrum Sativum L. in albino rats

ABSTRACT

This work has been carried out in order to investigate the possible ameliorative effect of Coriander in arsenic - induced toxicity in albino rats. 2,3 Dimercaptosuccinic acid [DMSA] was used as chelating agent. To achieve this purpose, 60 adult albino rats were used and randomly divided into equal 5 groups [12 rats each]. Group 1 served as negative control, group 2 received daily 100 ppm of sodium arsenite in drinking water, group 3 rats received daily oral dose of sod. arsenite and Coriander seeds extract [CSE] [1 g/kg body weight]., group 4 rats received sod. arsenite and oral dose of DMSA [0.05 g/ kg b.w] for 5 days lasted. Group 5 rats received sod. arsenite, Coriander seeds extract [CSE] and DMSA for 5 days lasted. The experiment lasted for 8 weeks and blood samples were withdrawn 4 and 8 weeks after induction of toxicity and different treatments. The antioxidant potential was carried out using the methanolic extract of Coriander seeds. Reducing power assay and Diphenyl picrylhydrazyl [DPPH] radical scavenging assay methods proved the antioxidant potency of CSE. In-vivo studies using the different biochemical techniques and antioxidant parameters proved that sodium arsenite caused a significant increase in all parameters concerned the liver toxicity while treatment of CSE or DMSA caused an ameliorative effect on this toxicity. Induction of hepatotoxicity by arsenite together with CSE and DMSA proved the synergistic effects of these chelating agents on arsenite toxicity