Simultaneous analysis of multidrug resistance 1[MDR1] C3435T, G2677T/A, and C1236T genotypes in Hamadan city population, west of Iran

ABSTRACT

One of the limitations in the treatment of common diseases such as cancer chemotherapy is development of multidrug resistance [MDR]. Polymorphisms could alter the expression level of MDR1 gene, which plays an important role in MDR. In this research, the frequency of C3435T, C1236T, and G2677T/A polymorphisms of MDR1 gene was investigated in a large group of population from Hamadan city to provide a sample data resource.Peripheral blood [2 ml] was taken, and DNA extraction was carried out. Multiplexed mutagenically separated PCR, which was followed by polyacrylamide gel electrophoresis and silver staining, was applied to detect the mentioned polymorphisms in 935 individuals. Sequencing performed for confirmation of gel electrophoresis resulted in 10 random cases. In total, alleles and genotypes of 933 persons [776 women and 157 men] were determined. The most frequent alleles of the polymorphisms were 3435T, C1236, and G2677. The most frequent genotypes were 3435C/T, 1236C/T, and 2677G/A, and their concurrent presence was also found as the most frequent simultaneous genotypes. There was not any meaningful difference among the prevalence of these genotypes in groups of men and women. Our results were close to those of other studies performed in Iran and compared to the other ethnic groups, which showed more similarity to Asian peoples than Europeans. As an aspect of personalized medicine, it could be used by chemotherapists to improve the routine methods of cancer treatment.