[Study of the anti bacterial effect of the pericarps of many varieties of punica granatum on E.coli biotype [1] which reveal resistance toward many antibiotics]

ABSTRACT

The Search for new antibiotics is the problem of resistance's bacteria to antibiotics. This research aims to investigate the effectiveness of anti-bacteria shown by the extracts prepared from per carps of different types of Punica granatum [L] [from the family Punicaceae], which grow in Syria against Escherichia coli [E.coli] biotype [1], which were resistant to studied antibiotics. Investigation began for E.coli in 843 milk samples, using blood agar, MacConkey agar, salmonella - shigella agar, and biochemical testing method [API 20 E = identification system for Enterobacteriaceae]. Then per carps of different types of Punica granatum were extracted by water, absolute alcohol, and eter using soxhlet device and rotary vacuum evaporator. Antibiotic susceptibility testing for E.coli by Kirby-Bauer disk diffusion method were conducted .then extracts susceptibility testing for E.coli were studied. Escherichia coli Type [1] was 33.35% of the total number of samples. This study also has shown the presence of antibacterial effectiveness of the extracts prepared from per carps of different types of Punica granatum against E.coli biotype [1], whereas the studied antibiotics have shown any antibacterial effectiveness. From the above we recommend to study the impact of Punica granatum all types of other microbial, which have resistant to antibiotics, also we recommend to study the active ingredients which have the antibiotic effect ,and determine their concentrations to be able to find suitable alternatives and effective in the treatment of infectious diseases