Evaluation of Anti- HLA Class I antibodies in chronic rejection of kidney transplantation

ABSTRACT

Studies have shown that patients who do not produce donor specific and / or panel reactive anti-HLA antibodies have a longer graft survival. The purpose of this study was to evaluate the posttransplant humoral immune response towards HLA-class I antigens and the measurement of the serum creatinine levels which are used in monitoring posttransplant function of kidney. Serum samples from 132 renal transplant recipients were screened for preformed anti-HLA class I panel reactive antibodies [PRA] by means of microlymphocytotoxicity assay. The results revealed the presence of PRA in 26 [19.7%] out of 132 transplanted patients. Graft function was evaluated by measurement of serum creatinine levels which revealed the mean of 1.75 mg/di [SD 1.08]. Because of clinical significance of presence of different PRA amounts [>10%, > 20% and >50% of panel reactivity] in patients, correlation with kidney function status was analyzed. The obtained data highlighted a higher presence of serum creatinine levels in PRA-positive patients compared to negative patients [P<0.01]. These results [and further studies for class II, ...] can be used to implement new therapeutic strategies to curtail post transplant alloantibodies production and better allografts survival