Comparison of four DNA extraction methods for the detection of Mycobacterium leprae from Ziehl-Neelsen-stained microscopic slides

Jenny Laura, Ruiz Fuentes; Alexis, Diaz; Anayma Elena, Entenza; Yahima, Frion; Odelaisy, Suarez; Pedro, Torres; Yaxsier, De Armas; Lucrecia, Acosta

Jenny Laura, Ruiz Fuentes; Alexis, Diaz; Anayma Elena, Entenza; Yahima, Frion; Odelaisy, Suarez; Pedro, Torres; Yaxsier, De Armas; Lucrecia, Acosta.

International Journal of Mycobacteriology. 2015; 4 (4): 284-289

in English | IMEMR | ID: emr-173959

ABSTRACT

ABSTRACT

Objective/

background:

The diagnosis of leprosy has been a challenge due to the low sensibility of the conventional methods and the impossibility of culturing the causative organism. In this study, four methods for Mycobacterium leprae nucleic-acid extraction from Ziehl- Neelsen-stained slides [ZNS slides] were compared Phenol/chloroform, Chelex 100 resin, and two commercial kits [Wizard Genomic DNA Purification Kit and QIAamp DNA Mini Kit]

Subject(s)

DNA; Staining and Labeling; Genomics; Polymerase Chain Reaction; Leprosy/diagnosis

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Index: IMEMR (Eastern Mediterranean) Main subject: Staining and Labeling / DNA / Polymerase Chain Reaction / Genomics / Leprosy Language: English Journal: Int. J. Mycobacteriology Year: 2015

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