use of micro-technique in serogrouping of E coli strains

ABSTRACT

A micromethod for serogrouping Escherichia coli strains, using microtitre set is described. The optimum agglutination reaction was detected and found to be with the test volume 0.05 ml of each of antigen and antiserum [diluted 180] combination. Because of the small amount of antiserum used in this method, it is practical to screen unknown strains directly with individual antisera. The method was found simple, accurate and economic in terms of time, antisera and reagents utilized in the test