role of the intensive care unitenvironment and health-care workers inthe transmission of bacteria associatedwith hospital acquired infections

ABSTRACT

The goal of this study was to attempt to determine the rate of con-tamination of health-care workers' [HCWs] hands and environmental surfaces inintensive care units [ICU] by the main bacteria associated with hospital acquiredinfections [HAIs] in Tehran, Iran. A total of 605 and 762 swab samples wereobtained from six ICU environments and HCWs' hands. Identification of the bac-terial isolates was performed according to standard biochemical methods, andtheir antimicrobial susceptibility was determined based on the guidelines recom-mended by clinical and laboratory standards institute [CLSI]. The homology ofthe resistance patterns was assessed by the NTSYSsp software. The most frequent bacteria on the HCWs' hands and in the environmental samples were Acinetobac-ter baumannii [1.4% and 16.5%, respectively], Staphylococcus aureus [5.9% and 8.1%,respectively], S. epidermidis [20.9% and 18.7%, respectively], and Enterococcus spp.[1% and 1.3%, respectively]. Patients' oxygen masks, ventilators, and bed linens werethe most contaminated sites. Nurses' aides and housekeepers were the most contam-inated staff. Imipenem resistant A. baumannii [94% and 54.5%], methicillin-resistantS. aureus [MRSAs, 59.6% and 67.3%], and vancomycin resistant Enterococci [VREs, 0%and 25%] were detected on the hands of ICU staff and the environmental samples,respectively. Different isolates of S. aureus and Enterococcus spp. showed significanthomology in these samples. These results showed contamination of the ICU environ-ments and HCWs with important bacterial pathogens that are the main risk factorsfor HAIs in the studied hospitals