Cloning and Optimization of Soluble Vascular Endothelial Growth Factor [165] Expression in Escherichia coli

ABSTRACT

Background: Vascular Endothelial Growth Factor [VEGF] is a coordinate regulator of physiological angiogenesis during embryogenesis, skeletal growth and reproductive functions. There are several types of VEGF, including VEGF [165] . VEGFs stimulate endothelial cell growth, angiogenesis, and capillary permeability. Low induction temperature is a major factor for expression of the recombinant VEGF [165] in soluble form. The purpose of this study was cloning and optimization of soluble vascular endothelial growth factor [165] expression in Escherichia coli [E. coli]