Sub-acute exposure to magnetic field induced apoptosis in thymus female rats
Pakistan Journal of Medical Sciences. 2005; 21 (3): 292-297
in English
| IMEMR
| ID: emr-176432
ABSTRACT
Apoptosis induced by magnetic field in female rats was investigated by using the Tdtmediated dUTP nick-end labeling [TUNEL] assay in thymus, liver and kidney. Design:
Female.s rats were exposure to 128 mT 1hour/day for 10 consecutive days. Small pieces of thymus, liver and kidney were fixed overnight at room temperature by direct immersion in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4. The samples were dehydrated with ethanol and toluene and embedded in paraffin wax. Serial sections [5 microm thick] were mounted on gelatin-coated glass slides and stained with hematoxylin and eosin or treated with TUNEL [TdT-mediated dUTP-digoxigenin Nick and Labeling] method.Setting:
Female Wistar rats were housed in a cage, with free access to food and water [Faculté des Sciences de Bizerte, Tunisia]. Rats were cared for under the Tunisian Code of Practice for the Care and Use of Animals for Scientific purpose and the Experimental Protocols were approved by the Ethics Committee.Subjects:
Treated and control groups [n=12] weighing 100-150g at the time of experiments were housed in the same condition three weeks before the beginning of the experiments. Main outcomemeasures:
MF-induced apoptosis in thymus was demonstrated by the terminal deoxynucleotidyl transferase [TdT] mediated dUTP-Biotin Nick End Labeling [TUNEL] assay.Results:
Following sub-acute exposure to MF, morphological examinations revealed numerous apoptotic cells in thymus characterized by nuclear fragmentation and condensation [figure 2a]. Interestingly, no labeling was found in control thymus. The density of the apoptotic cells was significant in cortical zone compared to control [2.95 +/- 0.34% vs 0.74 +/- 0.10%, p<0.05], than in the medullar zone [2.105 +/- 0.356% vs 0.634 +/- 0.038%, p<0.05] [figure 2a, b]. By contrast, as shown in figures [3c, d], no labeling was found in liver and kidney following MF exposure [figure 4e, f].Conclusions:
Thus, it may be concluded that static magnetic field induced apoptosis in thymic cell death but not in the liver and kidney
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Index:
IMEMR (Eastern Mediterranean)
Language:
English
Journal:
Pak. J. Med. Sci.
Year:
2005
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