Optimization of RNA extraction from rat pancreatic tissue

ABSTRACT

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction 1] homogenizing, 2] effective denaturation of proteins from RNA, 3] inactivation of ribonuclease, and 4] removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of RNase. Various technical difficulties occur during extraction of RNA from pancreatic tissue due to spontaneous autolysis. Since standard routine protocols yield unacceptable results in pancrease, we have designed a simple method for RNA extraction by comparing different protocols