Enhancement of sodium taurocholate to the absorption of cefquinome

ABSTRACT

The objective of the research was to detect the enhancement effect of sodium taurocholate on the absorption of cefquinome both in Caco-2 cells and rats. The absorption efficiency of cefquinome was determined by high performance liquid chromatography and calculated with apparent permeability coefficients [Papp] after Caco-2 cell monolayers treated odium taurocholate [2 mmol/L] and cefquinome. The results showed that the absorption of cefquinome in Caco-2 cell monolayers was significantly increased with the sodium taurocholate [2mmol/L]. Similar results were also detected in the rats orally administrated with 1 mL PBS of cefquinome [20mg/mL] containing different concentration of sodium taurocholate [5 mmol/L, 10mmol/L and 20mmol/L] respectively. Compared with control group, sodium taurocholate at 10 and 20 mmol/L increased the absorption of cefquinome in rats from 0.26 +/- 0.04micro g/mL to 0.57 +/- 0.03micro g/mL, 0.78 +/- 0.07micro g/mL respectively. These results indicated that sodium taurocholate could increase the intestinal permeability in a concentration-dependent mode, which will be useful for clinical treatment