Effects of spironolactone towards rabbit atrial remodeling with rapid pacing

ABSTRACT

This study aimed to observe the effects of spironolactone towards the rabbit atrial remodeling with rapid atrial pacing [RAP]. 30 rabbits were randomly divided into control group, RAP group and spironolactone group, with 10 rabbits in each group. RAP was performed at the speed of 800 beats/min for 8 h, atrial effective refractory period [AERP] was determined before and at the 1[st], 2[nd], 4[th], 6[th] and 8[th] of the pacing, the expressions of atrial muscular calcium channel alpha 1C subunit and beta 1 subunit mRNA were performed the RT-PCR detection, and ultrastructural changes of atrial myocytes were observed. AERP of RAP group shortened, with poor frequency adaptability; the expressions of calcium channel alpha 1C subunit and beta 1 subunit mRNA decreased 22% and 26%, respectively, when compared with the control group; ultrastructure of atrial myocytes changed significantly. AERP of spironotlactone group shortened less that RAP group, and the frequency adaptability was maintained, the decreased expressions of calcium channel alpha 1C subunit and beta 1 subunit mRNA significantly reduced. RAP could cause atrial remodeling, while spironolactone could inhibit RAPinduced atrial remodeling