Experimental induction of hepatitis C virus in Wister albino rat

ABSTRACT

Ten Wister albino rats were used in this Experiment. 6 animals were injected intra-peritonially by 0.2 ml of Hepatitis C patients blood, while 4 animals were injected with 0.2 ml of Healthy [non- infected] persons and kept as controls. They were devided into 3 groups. The first group consisting of 2 test animals and one control were slaughtered and autopsied at 2 months of injection. The second group were 2 test animals and one control were autopsied at 4.5 months after being injected. The third group were 2 animals and one control autopsied at 5 months after injection. The postmortem .picture in the first group showed diffuse cloudy swelling of the Liver cells with proliferation of Kuppfer cells in the portal tracts which is a picture of acute hepatitis. The second group showed marked cloudy swelling of the liver cells with hypertrophy of Kuppferts cells in the portal tracts. The liver lobule near the central vein were free of fat. The third group showed chronic hepatitis with marked cloudy swelling parenchymal necrobiosis in the form of Rim pycnosis caryohexis karyopycnosos and finally karyolysis. There were marked perplasia of hepatocytes which in some places produced rounded nodules that lost the radial cord arrangement producing preneoplastic nodules that may be after a time proceed to hepatocellular carcinoma