Dimethyl 3, 3', 4, 4'-tetrahydroxy-delta-truxinate isolated from the leaves of Andrographis lineata.Wall. ex. nees suppress adipogenesis in 3T3-L1 preadipocytes for type 2 diabetes

ABSTRACT

The present investigation elucidates the isolation and characterization of bioactive compound from the ethanolic leaf extract of Andrographis lineata [EtALL] which suppress the differentiation of 3T3-L1 adipocytes. The ethanolic leaf extract was subjected to bioassay guided fractionation in 3T3-L1 cell lines. Five fractions were isolated from the EtALL extract by column chromatography. All the Fractions [I-V] along with EtALL were screened for adipogenesis activity [Oil-Red-O staining].The fraction which showed maximum adipogenesis activity was purified by thin layer chromatography. The bioactive Fraction IV was found to have maximum adipogenic [96.83%] activity and the activity was comparable to Rosiglitazone. The spectroscopic data analysis reveals that, the isolated bioactive compound was Dimethyl 3, 3', 4, 4'-tetrahydroxy-delta-truxinate [DT[delta]T], a combination of truxillic and truxinic acid derivative. DT[delta]T showed insulin mimicking [131.2%], sensitizing [810.02%] and adipogenic activity [80.23%]. Hence our present study concluded that, Dimethyl 3, 3', 4, 4'-tetrahydroxy-delta-truxinate isolated from the ethanolic leaf extract of Andrographis lineata stimulates glucose uptake, potentiates insulin-stimulated glucose in 3T3-L1 adipocytes without increasing adiposity