Detection of stability and degradation of piperacillin and tazobactam in injectables from in-patient wards and pharmacy by RP-HPLC method

New RP-HPLC method for the detection of degradants and quantification piperacillin and tazobactam in injectables stored in inpatient wards and pharmacy has been developed on C[8] column [250 x 4.6, 5 micron] using methanol and water [55:45% v/v] as mobile phase and Diode array detection at 215 nm. Linearity regression coefficients were more than 0.999 and % RSD for intra- and inter-assay precision and accuracy were less than 2. Selectivity of the method for all possible degradants and analytes were established by mild acidic and alkaline stress degradation using 0.001M HCl and 0.001M NaOH. Method was applied on various samples of injectables collected from inpatients wards and pharmacy. Results revealed that few minor degradants were observed in samples collected from refrigerator and 9 degradants were found in samples collected from trays of inpatient ward. Formed degradants were identical with acid/base hydrolytic products of stress studies. This RP-HPLC method is highly reliable in hospitals and clinical analysis of Piperacillin and Tazobactam in Injectables to preserve potency, to prevent resistance and to ensure efficacy. This study educates the paramedical staff in handling and storage of Piperacillin and Tazobactam in injectables in wards and pharmacy stores