<A> nested-splicing by overlap extension PCR improves specificity of this standard method

Ali Asghar, Karkhane; Bagher, Yakhchali; Ferdous Rastgar, Jazii; Bijan, Bambai; Saeed, Aminzadeh; Fatemeh, Rahimi

nested-splicing by overlap extension PCR improves specificity of this standard method

Ali Asghar, Karkhane; Bagher, Yakhchali; Ferdous Rastgar, Jazii; Bijan, Bambai; Saeed, Aminzadeh; Fatemeh, Rahimi.

IJB-Iranian Journal of Biotechnology. 2015; 13 (2): 56-59

in English | IMEMR | ID: emr-179812

ABSTRACT

ABSTRACT

Background:

splicing by overlap extension [SOE] PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein's structure and function

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Index: IMEMR (Eastern Mediterranean) Language: English Journal: Iran. J. Biotechnol. Year: 2015

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Index: IMEMR (Eastern Mediterranean) Language: English Journal: Iran. J. Biotechnol. Year: 2015