Some properties of the extracellular, cell bound and intracellular protease of mucor hiemalis

ABSTRACT

This study was undertaken to provide some basic aspects of the extracellular, intracellular and cell-bound protease of M. Hiemalis. was also tested for the production of milk-clotting enzymes. It was found that the protease whether obtained extracellularly or intracellulary exhibited milk-clotting action, although it was very weak and took about 8 hours to clot the milk. Intracellular protease showed maximum activity when it was extracted with 7.8 pH phosphate buffer. It was most active when incubated at 35°C. The protease hydrolysed casein and the rate of hydrolysis increased with the increase in time. Similar results were obtained with the extracellular and cell-bound protease