Analysis of hsp90a gene expression induced by temperature shock and heavy metals in the heart of common carp [Cyprinus Carpio L.]

ABSTRACT

This study was conducted to analyze the hsp90a gene expression in the heart of carp [Cyprinus carpio] in vivo during exposure to heavy metals [Arsenate, cadmium and copper] and changes in temperature. hsp90a gene induction were measured in the heart by semiquantitative RTPCR. Heat shock resulted in a transient induction of hsp90a [15-fold], while cold shock treatment induced high level [26-fold] of hsp90a expression. Among heavy metals, arsenate proved to be the most potent inducer of hsp90a gene. The hsp90a mRNA levels were elevated 11-fold after 24- h exposure, compared to control. Cadmium at 10 mg/L had no impact on the expression of hsp90a; while cadmium, at 10mg/kg, resulted in significant induction of hsp90a gene [14.5-fold and 17.5- fold] respectively, at 24 h and 48 h exposure. The various induction patterns suggest that hsp90a gene play different role in cardiac protection.