Presence of bla[PER-1] and bla[VEB-1] beta-lactamase genes among isolates of Pseudomonas aeruginosa from South West of Iran

ABSTRACT

Pseudomonas aeruginosa isolates have acquired resistance to antibiotics such as novel beta-lactams. The aim of this study was to investigate the bla[PER-1], bla[VEB-1], and bla[PSE-1] genes among isolates of P. aeruginosa among intensive care unit [ICU] patients. Sixty-five isolates were collected. The antibiotic susceptibility testing and combined disk tests were performed to detect the isolates producing extended spectrum beta-lactamases [ESBLs] among ceftazidime-resistant isolates. Polymerase chain reaction [PCR] amplification of blaPER-1, blaVEB-1, and blaPSE-1 genes was conducted. Ten [15.3%] isolates were ESBL-positive, of which 40% [n = 4] belonged to males and 60% [n = 6] were collected from females. Moreover, two and one isolates harbored blaPER-1 and blaVEB-1 genes, respectively