efficacy of non cultured melanocytes and keratinocytes transplantation in treatment of generalized vitiligo

ABSTRACT

Objective: determine the efficacy and safety of the non-cultured melanocyte-keratinocyte transplantation procedure in the treatment of stable resistant generalized vitiligo. Design: A simpler and modified method based on that of Olsson and Juhlin [1998] was performed this method uses a shaved biopsy skin sample one tenth the size of the recipient area. The skin sample is incubated, and the cells are mechanically separated using trypsin-EDTA solution and then centrifuged to prepare a suspension. Cell suspension is then applied to the derma braded depigmented skin area, and a collagen dressing is applied to keep it in place