Testing of enzyme linked immunosorbent assay [ELISA] in the diagnosis of pemphigus

ABSTRACT

The aim of this study was to evaluate the diagnostic value of Dsg1 and 3 enzyme-linked immunosorbent assay [EL1SA] in the diagnosis of pemphigus. Thirty-six patients with pemphigus were enrolled. The diagnosis was confirmed by histopathology and direct immunofluorescence [DIF]. Autoantibodies against Dsg1, and Dsg3 were assayed, by ELISA, in serum of pemphigus patients. In pemphigus vulgaris [PV], anti-Dsg1 and anti-Dsg3 antibodies titer were raised above the cut off values 88.5% and 96.1% of patients respectively. Dsg-3 was more sensitive than Dsg-1[96.2% 88.5%] while both be the same specificity 95%. In PF and PH, the only anti-Dsg1 level was in all cases [100%]. Conclusion: In PV Dsg-3 was more sensitive than Dsg1 while in and pemphigus herpetiformis, only anti-Dsg-1 was detected in all cases [100%]. Both anti-Dsg1Ab and anti-Dsg3Ab ELISA values are correlated with the disease activity