Light and electron microscope study of the diabetic rat myocardium after trimetazidine [vastarel] treatment

ABSTRACT

Diabetic patients with ischaemic heart disease have a greater liability of myocardial ischaemia, and an increased incidence of heart failure compared to the non-diabetic ones. The goal of this study was to clarify the effects of trimetazidine on the architecture of the myocardium of diabetic rats. Thirty adult male albino rats [200-250 gm] were used in this investigation. They were divided into three equal groups; control diabetic non treated and diabatic TMZ- treated. At sacrifice, small pieces of the myocardium of left ventricle were processed for histological, histochemical and immunohistochemical study. Myocardium of diabetic rats showed an apparent increase of endomysium.The muscle fibers showed areas of degeneration. Ultrastructurally, the cardiac myocytes of diabetic rats showed distortion of cardiac myofibrils with loss of cross banding in many areas. The nucleus had a corrugated nuclear membrane and the mitochondria were swollen and distorted. Histochemically, myocardium of diabetic rats exhibited a weak succinic dehydrogenase reaction and a strong positive immunoreaction for NK-kappa B and caspase-3 in myocardial sarcoplasm. On the other hand, TMZ- treated diabetic rats showed an improvement in the histological architecture and in both histochemical and immunohistochemical reaction. So, TMZ should always be advised for diabetic patients to alleviate the cardiac hazards