[Investigation of the interaction between L929 cell line with human 3D skin matrix]

ABSTRACT

Background and Aim: Ideal scaffold for tissue engineering must be a mimic of extracellular matrices that facilitates proliferation and differentiation of the cells. Biological scaffolds are prepared from decellularization of human or animal tissues. Extracellular matrix of natural tissues can be used as a scaffold for regenerative applications, even xenogenically. In this study, for the first time complete human skin tissue [dermis plus epidermis] was decellularized. This matrix can be used in the clinics for the treatment of severe burns. Unseparated epidermis from the dermis may cause the skin to maintain higher levele of moisture until complete healing