[Site directed mutagenesis in ifnbeta gene in order to increasing its mRNA stability and translation level using megaprimer PCR method]

ABSTRACT

Background and Objectives: Beta interferon [IFNbeta] protein is produced as a recombinant drug and used in treatment of some diseases like Multiple Sclerosis. In eukaryotic cells, IFNbeta mRNA is rapidly degraded and its halflife is too short. One of the contributing factors to this short half-life is presence of the AU rich element [ARE] in 3'UTR of this mRNA. This region has an inhibitory effect on translation too. Our aim in this research was to delete ARE from IFNbeta gene in order to increase its mRNA stability and translational level