Anti-inflammatory effects and mechanisms of Hizikia fusiformis via multicellular signaling pathways in lipopolysaccharide-induced RAW 264.7 cells
Pakistan Journal of Pharmaceutical Sciences. 2017; 30 (1): 43-48
in English
| IMEMR
| ID: emr-185738
ABSTRACT
In this study we investigated the anti-inflammatory effects and mechanisms of Hizikia fusiformis [HF] extracts in lipopolysaccharide [LPS]-induced RAW 264.7 cells. We extracted HF using solvent and sub-critical water techniques. In results, HF extracts inhibited nitric oxide [NO] production in cell-free and LPS-stimulated RAW 264.7 cells. HF210 [extract prepared with sub critical water at 210[degree]C] was most effective. The HF210 extract dose-dependently inhibited inducible nitric oxide synthase expression [iNOS] and nuclear factor kappa [NF-kB] p65 translocation from cytosol to the nucleus. Furthermore, HF210 extract dose-dependently inhibited the phosphorylation of p38 mitogen-activated protein kinase [p38 MAPK], Jun N-terminal kinase [JNK], and signal transducers and activators of transcription [STAT]-1in LPS-induced RAW 264.7 cells. Thus, our results suggest that anti-inflammatory effects of HF210 extract showed a noticeable distinction by regulation of multiple signaling pathways in LPS-induced RAW 264.7 cells
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Plant Extracts
/
Signal Transduction
/
Lipopolysaccharides
/
NF-kappa B
/
Phaeophyta
/
RAW 264.7 Cells
/
Macrophages
Language:
English
Journal:
Pak. J. Pharm. Sci.
Year:
2017
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