role of Coenzyme Q[10] on the Total Antioxidant Capacity of Mouse Vitrified Pre-Antral Follicles

ABSTRACT

Occurrence of oxidative stress [OS] following in vitro culture is inescapable. Therefore, the aim of the present study was to determine the efficacy of Coenzyme Q[10] [CoQ[10]] supplementation medium on developments and Total Antioxidant Capacity [TAC] of mouse vitrified preantral follicles. Fresh and vitrified-warmed isolated preantral follicles from ovaries of 14-16 day-old mice were cultured in supplemented alpha- MEM medium with or without CoQ[10]. On the twelfth day of culture period, ovulation was induced by adding 1.5 IU/ml Human Chorionic Gonadotropin [hCG]. Rates of survival, antrum formation and developmental stages of released oocytes [GV, MI, MII] were evaluated. Separately, Total Antioxidant Capacity [TAC] levels were measured at initial time, 24, 48, 72 and 96 hours of culture period by Ferric Reducing/Antioxidant Power [FRAP] assay. The results showed that the mean diameter and rates of survival, antrum formation, ovulation and MII oocytes of fresh and vitrified-warmed isolated preantral follicles with pretreatment of CoQ[10] were significantly higher than those of respective groups without pretreatment of CoQ[10]. TAC levels significantly increased up to 96 hours in fresh and vitrified-warmed preantral follicles with pretreatment of CoQ[10] compared with those which were cultured without CoQ[10]. Supplemented culture medium with CoQ[10] promotes TAC levels and development of both fresh and vitrified-warmed isolated preantral follicles