Fate of incorporated 1,4-C 14 succinic acid in corn seedlings grown under saturated atmosphere of Co2 and normal conditions

ABSTRACT

Radioactivity distributions corn seedlings incubated in saturated atmosphere of CO2 -for 3 hours, and after transferring to normal condition for intervals 1,3 and 5 hours, after 2 C[14]alanine incorporation, was investigated. An increase in amino acids and sugars radioactivities was observed, with significant decrease in organic acids. Saturated atmosphere of Co2 leads to disorder in the function of kreb's cycle, which appeared as radioactivity accumulation in succinic acid and decrease the radioactivity of citric, malic, aconitic, and fumaric acids. Saturated atmosphere of Co2 affect in succinic acid dehydrogenase activity, which consequently lead to blok alanine oxidation to pynvie acid, and then through acids of kreb's cycle. Among amino acids, the radioactivity accumulation was detected in glutamic, alpha-amino butyric acid [10 folds], as compared to control. After 5 hours of transferring the seedlings to normal condition, the radioactivity of aspartic, asparagines, glutamic acids, and glycine + serine was returned to control level