Application and evaluation of the immunofiltration assay in the diagnosis of human schistosomiasis

ABSTRACT

In a trial to reach fast, simple and efficient diagnostic assay for schistosomiasis, Dot Dye Immunofiltration assay using protein A conjugated gold colloid was applied and evaluated in comparison to Dipstick assay and Enzyme Immunoelectrotransfer Blot Assay [EITB], Both soluble egg antigen [SEA] of Schistosorna mansoni and Schistosoma haematobium were used as capture antigens in each assay. Dot Dye Immunofiltration assay, Dipstick assay and EITB were found to be more efficient by using SEA of S. mansoni, than by using SEA of S. haeinatobium. Using SEA of S. mansoni, Dipstick assay was found to be the most efficient [82.2%] among all performed assays followed by Dot Immunofiltration assay and EITB [80.5%] for each. On the other hand Dot Dye Immunofiltration assay was found to be simpler and faster than Dipstick assay and EITB