Production of the phospholipase D and heat-shock protein [HSP]-60 recombinant proteins from Corynebacterium pseudotuberculosis

ABSTRACT

Background: Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, is one of the most important diseases of sheep and goats, causing considerable losses for herd owners. Phospholipase D [PLD] is a potent exotoxin produced by C. pseudotuberculosis and it has been considered as the major virulence factor for this bacterium, possibly contributing to the spread of the bacteria from the initial site of infection to secondary sites within the host. Heat shock proteins [HSPs] are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune re-sponses in mammals