Comparison of three routine protein precipitation methods: acetone, TCA/acetone wash and TCA/acetone

ABSTRACT

The aim of this study was to compare three routine precipitation methods, including acetone, TCA/acetone wash and TCA/acetone. 30 plasma samples were precipitated using above mentioned three methods. Pellets were dissolved in rehydration solution and protein quantification was done for this solution. According to statistical analysis using SPSS ver 16.0 software and one-way ANOVA test, the protein yield of acetone method was greater than two other methods and was statistically different from TCA/acetone precipitation method [p-value 0.006]. The acetone method is more efficient than two other methods in protein precipitation