DNA sequencing and molecular modelling of HLA-B 27 gene sub types in patients with acute anterior uveitis and ankylosing spondylitis

ABSTRACT

HLA-B 27 is a human leukocyte antigen [HLA] class I cell surface molecule located on the short arm of chromosome 6. It is strongly associated with Acute anterior uveitis [AAU] and Ankylosing spondylitis [AS], the disorders are not only by genetic inherited disease. Several genetic and environmental factors likely play a vital role in determining the risk of developing these disorders. In this study, we try to find out the structure and functional relationship of HLA-B 27 sub types [HLA B[asterisk]27112, HLA B[asterisk]270401, HLA B[asterisk]2706, HLA B[asterisk]270502]. The molecular modeling [3D structure] of these subtypes are constructed and ligand binding sites are predicted. The HLA-B 27 gene were amplified from the DNA isolated from the patients with AS and AAU are sequenced. The protein sequences of the HLA-B 27 subtypes obtained from the IMGT/HLA database are aligned with each other and structure of all the subtypes models were constructed by in-silico method. The binding sites of the HLA-B 27 protein and their subtypes was predicted using 3DLigandSite server. The accurate prediction of ligand binding sites on the HLA-B 27 protein surface can be very helpful for rational drug design of AAU and AS