Molecular characterization of ciprofloxacin resistant e. coli in community acquired urinary tract infection

ABSTRACT

Widespread use of ciprofloxacin in treatment of urinary tract infection [UTI] led to increased level of resistance in clinical isolates of E. coli. The aim of this study was to investigate the molecular characterization of ciprofloxacin resistant E.coli isolates from community acquired urinary tract infections.. E-coli strains were isolated from urine samples [E. coli represented 70% of isolates [21/30] and minimal inhibitory concentration [MIC] of Ciprofloxacin [CFX] of E. coli was measured [Resistant strains of E. coli had MICs of ciprofloxacin ranging from 4 to >32 mg per liter]. CFX resistant E. coli strains were subjected to PCR to amplify gyrA and parC genes of Quinolones resistance determining region of E. coli. DNA sequencing of amplified product was carried out and the molecular characterization of E. coli resistant to CFX were analysed [All resistant E. coli isolates contained Ser83+/-Leu and Asn87+/-Asp mutations in gyrA and a Ser83-+/-Ile mutation in parC; two isolates also contained a Glu84+/-Val mutation in parC.