Expression of MLAA34-HSP70 fusion gene constructed by SOE-PCR
Pakistan Journal of Pharmaceutical Sciences. 2017; 30 (3 [Special]): 1125-1127
in English
| IMEMR
| ID: emr-189323
ABSTRACT
To construct the pIRES2-MLAA34-HSP70 recombinant vector and express the MLAA34-HSP70 recombinant proteins in Escherichia coli [E. coli]. The MLAA34 and the HSP70 genes were extracted from U937 cells by RT-PCR, and then we amplified the fusion gene MLAA34-HSP70 by SOE-PCR and inserted it into the pIRES2-EGFP vector to construct the pIRES2-MLAA34-HSP70 recombinant vector. We amplified the fusion gene MLAA34-HSP70 successfully and identified the correctness of pIRES2-MLAA34-HSP70 recombinant vector by PCR and restriction endonuclease. Moreover, the MLAA34-HSP70 recombinant proteins expressed in E. coli were consistent with the expected molecular weight. We constructed the pIRES2-MLAA34-HSP70 recombinant vector successfully and the MLAA34-HSP70 recombinant proteins were successfully expressed by the induction of IPTG
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polymerase Chain Reaction
/
Vaccines, DNA
/
Artificial Gene Fusion
/
Escherichia coli
/
Apoptosis Regulatory Proteins
/
Gene Fusion
/
Antigens, Neoplasm
Language:
English
Journal:
Pak. J. Pharm. Sci.
Year:
2017
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