Detection of AD-BMP-2-IRES-HIF-1alpha MU on local promoting angiogenic and osteogenic capacity of necrosis area

ABSTRACT

The induced EPCs were transfected by Ad-BMP-2-IRES-HIF-1alphamu, and then transplanted into femoral head necrotic zone, the effect on osteogenesis and agiogenesis of necrosis zone was detected. The Ad-BMP-2-IRES-HIF-1alpha was transfected into induced EPCs and then transplanted into avascular necrotic parts of the femoral head [ANFH].Afterwards, the promotion effect on angiogenic and osteogenic capabilities of the necrosis parts from Ad-BMP- 2-IRES-HIF-1alpha was detected. Rabbit bone marrow MNCs were obtained by density gradient centrifugation method, and were induced into EPCs by M199 medium; EPCs were identified in accordance with the cell morphology, specific surface markers and uptake abilities. The Ad-BMP-2-IRES-HIF-1alpha was transfected to EPCs and then transplanted into parts of ANFH. The models were euthanized 2 and 4 weeks after operation and then the angiogenic and osteogenic indexes of necrotic parts were detected. The results showed that more blood vessels generated in group A than that in group B and C [P<0.05], and the statistical differences were found between group B and C [P<0.05]. The detection of histology and BMP-2 immunohistochemistry showed that there were statistically significant differences between group A and B, group A and C [P<0.05]. There was no significant difference between group B and C [P<0.05]. To sum up, this experiment shows that the EPCs transfected by Ad-BMP-2-IRES-HIF-1alpha have stronger angiogenic and osteogenic capabilities