Comparison of oxacillin and cefoxitin for the detection of mecA gene to determine methicillin resistance in coagulase negative staphylococci [CoNs]
JCPSP-Journal of the College of Physicians and Surgeons Pakistan. 2017; 27 (8): 520-522
in English
| IMEMR
| ID: emr-191058
ABSTRACT
The aim of the study was to compare the effectiveness of Cefoxitin with that of Methicillin/Oxacillin in the determination of mecA gene in Methicillin resistant Coagulase-negative staphylococci [CoNS]. We assessed 57 CoNS isolates for mecA gene via PCR, which were subsequently subjected to Methicillin/Oxacillin and Cefoxitin disc diffusion test. These methods are simple, inexpensive and easily available compared to PCR despite less specificity. Out of 41 mecA positive species, 33 [80.5%] were resistant to Methicillin/Oxacillin. Cefoxitin-resistance was seen in all 41 [100%] mecA positive samples. Two [12.5%] mecA negative isolates of S.saprophyticus were Methicillin/Oxacillin resistant, but were Cefoxitin sensitive. Four [9.7%] isolates of S.saprophyticus, three [7.3%] of S.epidermidis species, and one [2.4%] S.haemolyticus that were mecA positive were sensitive to Methicillin/Oxacillin but resistant to Cefoxitin. Cefoxitin resistance provides a more accurate picture of mecA gene positivity as compared to Methicillin and Oxacillin
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Index:
IMEMR (Eastern Mediterranean)
Language:
English
Journal:
J. Coll. Physicians Surg. Pak.
Year:
2017
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