Synthesis, optimization and biological evaluation of [99m]Tc-digoxin as possible cardiac imaging agent

ABSTRACT

Heart imaging radiopharmaceuticals could improve the diagnostic value of routine heart scanning for detecting cardiac disorders. The aim of the study was to prepare high radiochemical purity [99m]Tc-Digoxin in a yield of about 98%. The optimal conditions for labelling were as follows 100microg of Digoxin, 2microg of SnCl2·2H2O, room temperature [25 +/- 1°C], reaction retention time of 30 min at pH 7. Under these conditions, the radiochemical yield of [99m]Tc-Digoxin reaches 98%. In vivo bio distribution was performed in normal Swiss Albino mice at different time intervals after administration of [99m]Tc-Digoxin.Scintigraphic study of [99m]Tc-Digoxin was performed in rabbits. The heart uptake of 99m Tc-Digoxin was sufficiently high and thus may be a potential myocardial imaging radiopharmaceutical applicable in cardiology