Helicobacter pylori detection in chronic gastritis: a comparison of staining methods
JAMC-Journal of Ayub Medical College-Abbotabad-Pakistan. 2011; 23 (2): 112-114
in English
| IMEMR
| ID: emr-191819
ABSTRACT
Background:
Helicobacter pylori is an important cause of chronic gastritis, gastric ulceration and gastric malignancies as gastric carcinoma and MALT lymphoma. Its definitive diagnosis is based on histopathology. Routine H and E stain is not very effective in its detection, immune-stains and fluorescent stains are costly. Need for simple cheap and sensitive stain has always been a topic of hot debate and extensive research.Method:
paraffin embedded blocks of all adult patients diagnosed as chronic gastritis/gastric ulceration with no accompanying gastric pathology as hypertrophic gastropathys, and neoplasias were taken into study. Three sections of 4 micron were cut and stained with routine H and E, Giemsa, and Cresyl fast violet.Results:
Total number of patients was 50. Out of these 37 [74%] were males and 13 [26%] were females. Mean age of the patients was 50.4 years. Thirty-four percent [34%] were positive in normal H and E stain, 68% were positive in Giemsa and 76% were positive in Cresyl fast violet.Conclusion:
Cresyl fast violet is a good stain for diagnosis of H. pylori gastritis. Keywords H pylori, chronic gastritis, H pylori stainingmethods:
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Index:
IMEMR (Eastern Mediterranean)
Language:
English
Journal:
J. Ayub Med. Coll.-Abbotabad-Pak.
Year:
2011
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